CABELL.ANDREWS@BIONET-20.ARPA (04/04/88)
From: Paul Andrews <CABELL.ANDREWS@BIONET-20.ARPA> Douglas Kell:re-FAD synthesis with C. kluyveri Sorry I have been so long in replying, but I have been out of town for a while and haven't had a chance to check my mail. To remind you of my problem.....I am interested in synthesizing 2-C14-FAD from 2-C14-ribolflavin. One published procedure is to incubate Clostridium kluyveri with C14-riboflavinand then isolate C14-FAD from the cell pellet. I have had a few other suggestions from other groups and apparently the approach used now is to produce an "FAD synthetase" complex from Brevibacterium ammoniagenes. I am going to try this approach first before attempting to grow more bacteria. anyway, if I have anymore trouble I may be in touch. Thankyou for your responce. Yours truly (..et merci from a displaced Canadian...) Paul Andrews College of Pharmacy/Medicinal Chemistry University of Texas at Austin Austin, Texas 78712 (EMAIL CABELL.ANDREWS) -------