CABELL.ANDREWS@BIONET-20.ARPA (04/04/88)
From: Paul Andrews <CABELL.ANDREWS@BIONET-20.ARPA>
Douglas Kell:re-FAD synthesis with C. kluyveri
Sorry I have been so long in replying, but I have been out of town
for a while and haven't had a chance to check my mail. To remind you of my
problem.....I am interested in synthesizing 2-C14-FAD from 2-C14-ribolflavin.
One published procedure is to incubate Clostridium kluyveri with C14-riboflavinand then isolate C14-FAD from the cell pellet. I have had a few other
suggestions from other groups and apparently the approach used now is to
produce an "FAD synthetase" complex from Brevibacterium ammoniagenes. I am
going to try this approach first before attempting to grow more bacteria.
anyway, if I have anymore trouble I may be in touch. Thankyou for your
responce.
Yours truly (..et merci from a displaced Canadian...)
Paul Andrews
College of Pharmacy/Medicinal Chemistry
University of Texas at Austin
Austin, Texas
78712
(EMAIL CABELL.ANDREWS)
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