GEORGE@GUNBRF.BITNET (03/22/88)
From: <GEORGE%GUNBRF.BITNET@CUNYVM.CUNY.EDU>
To whom it may concern. Please, place this message on the Bionet
Bulletin Board.
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In reference to Roy Smith's (Public Health Research Institute) March 17
bulletin board notice. Rich Roberts' restriction enzyme list employs a
nonstandard ambigious nucleotide notation that was developed prior to the IUPAC
recommendations. As an alternative, Dr. Freidham Pfeiffer of the Max Planck
Institute for Biochemistry, Martinsreid FRG has been maintaining a set of
restriction enzyme lists for the past few years. These lists incorporate
information from Rich Roberts' collection and information compiled by Dr.
Kessler of Boehringer, Mannheim, FRG and conform to the standard IUPAC
recommendations. Dr. Pfeiffer's collection is separated into four files:
LONG.ENZ - containing all currently known enzymes; SHORT.ENZ - containing one
enzyme per unique recognition and cut site; AVAIL.ENZ - containing a list of
commercially available enzymes; and MERGED.ENZ - containing a list of all
enzymes on all of the other lists.
These lists are available in the public domain and are supplied by the Protein
Identification Resource (PIRMAIL@GUNBRF.BITNET), in conjunction with its
databases. The lists can also be directly obtained from Dr. Pfeiffer
(PFEIFFER@DM0MPB51.BITNET), from whom futher information may be obtained.
David G. George
Protein Identification Resource (PIR)
National Biomedical Research Foundation
Georgetown University Medical Center