EBEALE@BIONET-20.ARPA (03/31/88)
From: Elmus Beale <EBEALE@BIONET-20.ARPA> We are using transient expression assays to investigate the mechanisms that control cell-specific gene expression. We need a control vector that is efficiently expressed in all or most cell types. We have used enhancer/promoters from SV40, RSV, metallothionein, but these show tissue-preferences. Perhaps there is not a perfect control but I would think that the promoters from housekeeping genes such as tubulins, beta-actin, HPRT, or one of the histones might work. If anyone has suggestions, and (better yet) genomic clones of such genes please contact me. Thanks, Elmus Beale -------