elliston@av8tr.UUCP (Keith Elliston) (10/18/90)
Hello again... I recently posted a question on calculating protein extinction coefficients. I received a couple good replies, but I actually got the best information over the phone from Irv Edelman at GCG. He recommended a paper by Gill and von Hippel (Anal. Biochem. 182:319-26. 1989) which goes into the both the calculation on extinction coefficients at 280nm, as well as their significance WRT the real experimentally determined values. I then used their calculation (quite simple in fact) to write a little snippet of code that I put into the GCG program peptidesort. Now I have a little program that calculates extinction coefficients as well as all the other nice things peptidesort did before. If any one is interested in my little snippet of code, drop me a line and i can send it. According to Irv, Peptidesort will do these calculations in release 7, so don't feel that you need to modify your version right away ;) Thanks again for the info... Keith uunet!av8tr!elliston -- =============================================================================== Keith O. Elliston | .signature really under construction.... uunet!av8tr!elliston | ===============================================================================
MBCICERO%TWNAS886@PUCC.PRINCETON.EDU (10/22/90)
Hi, I am very interested.