bbaker@nmsu.edu (BAKER) (02/20/91)
I'm a senior at New Mexico State University, and in April I am required to present a seminar, on any topic i am remotely intersted in. As I'm interested in neuro/psychopharmacology, I thought I could talk about something like DNA synthesis, RNA translation, or protein function in models of learning and memory. Unfortunately, I'm having trouble finding any articles referencing this, although several professors have claimed this shouldn't be a problem... Can anybody out there tell me an article where i could start? Also, any sources of information on this topic would be GREATLY appreciated (although I could, I don't want to change my topic). Any advice? One more thing...this account i am posting from does NOT recieve email, so if anybody mails any responses, please send them to almartin@nmsu.edu Thanks all.... Brian Baker
hunter@work.nlm.nih.gov (Larry Hunter) (02/21/91)
As I'm interested in neuro/psychopharmacology, I thought I could talk about something like DNA synthesis, RNA translation, or protein function in models of learning and memory. Unfortunately, I'm having trouble finding any articles referencing this, although several professors have claimed this shouldn't be a problem... Well, there is not a great deal known about this topic yet, although there is some exciting research. I'm not an expert in the field, but I have tried to keep up with what's happening. I would recommend investigating two lines of research. One is Eric Kandel's work with the sea slug Aplysia. Try: TI - 5-HT modulates protein synthesis and the expression of specific proteins during long-term facilitation in Aplysia sensory neurons. AU - Barzilai A AU - Kennedy TE AU - Sweatt JD AU - Kandel ER SO - Neuron 1989 Jun;2(6):1577-86 AB - Long-term, but not short-term, facilitation induced by 5-HT in Aplysia sensory neurons is blocked by application of inhibitors of protein synthesis or RNA synthesis during the 1.5 hr training period. These findings suggest that genes and proteins not needed for the short-term process are required for long-term facilitation. To identify these proteins, we examined changes in overall and specific protein synthesis in sensory neurons. During the 1.5 hr period of stimulation, 5-HT initiated three temporally distinct changes in total protein synthesis: a small decrease at 0.5 hr, a small increase at 1 hr, and a second and larger increase at 3 hr. 5-HT also produced three temporally distinct changes in the synthesis of individual proteins that could be resolved on 2-D gels. First, there was an increase at 30 min in the rate of synthesis of 10 proteins and a decrease in the synthesis of 5 proteins. These changes in protein expression are rapid and transient, subsiding within 1-3 hr, and are transcription-dependent. Second, at 3 hr, there is a transient increase in 4 different proteins that also is transcription-dependent and lasts approximately 5 hr. Finally, at 24 hr, during the retention phase of facilitation, there was a sustained increase in the expression of two proteins. There are several follow up papers, but this is a good place to start. You also might want to look for the work of one of Kandel's students, Tom Carew. The other place to look is in the literature of cognitive defects introduced by mutations. There are a set of so-called "dunce" genes that have been identified in Drosophila, C. elegans, and other organisms. Try: TI - Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene. AU - Davis RL AU - Takayasu H AU - Eberwine M AU - Myres J SO - Proc Natl Acad Sci U S A 1989 May;86(10):3604-8 AB - A probe representing the Drosophila dunce+ (dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+ gene were isolated from rat libraries and characterized. This gene has been named ratdnc-1. One cDNA clone defines a large open reading frame of approximately 1.8 kilobases (kb), predicting a protein sequence of 610 amino acids with significant homology to a conserved domain of approximately 275 residues found in most other PDEases. The amino acid identity value to the Drosophila cAMP PDEase within this domain is a striking 75%. Other cDNA clones show blocks of sequence divergence from this cDNA clone close to the predicted N terminus, indicating the potential existence of a family of related enzymes encoded by alternatively spliced messenger RNAs from ratdnc-1. Genomic blotting experiments suggest the existence of at least one other rat gene with homology to ratdnc-1. RNAs homologous to ratdnc-1 are heterogeneous in size between tissues, with heart containing a major transcript of 4.4 kb and brain one of 4.0 kb. The potential identity of the product of the ratdnc-1 gene with known PDEases is discussed. I would also recommend chapter 8 of L.S. Seiden and L.A. Dykstra's "Psychopharmacology: A Biochemical and Behavioral Approach," (Van Nostrand, 1977) which, although somewhat dated, has a nice overview of the pharmacological and biochemical aspects of learning and memory. Hope this helps.... Larry Hunter (Another computer scientist interested in learning and memory in biological systems) -- Lawrence Hunter, PhD. National Library of Medicine Bldg. 38A, MS-54 Bethesda. MD 20894 (301) 496-9300 (301) 496-0673 (fax) hunter@nlm.nih.gov (internet) hunter%nlm.nih.gov@nihcu (bitnet/earn)