PC120@biology.cambridge.ac.uk (03/25/91)
Hello, I sent this to the INFO-GCG bulletin board at UTORONTO the other week, but didn't receive any replies. I'm giving it another go here.... >Hi out there! >I wonder if someone has a solution to this rather trivial problem.... >When I'm using SEQED compiling sequences of own constructs, e.g. new vectors >or vector/insert constructs, there's one thing that annoys me. >I haven't found out a straightforward way to include pieces of circular DNA >that spans over nucleotide #0; the program gets a blackout when you try to >include from, let's say 2435 to 29. It can of course be done in two steps, >but if you are working with the reverse strand, it really exhausts my >limited brain capacity to figure out where I am in the sequence and on >which strand. >Any advice? >Dr. Peter Carlsson >Dept. of Biochemistry >Univ. of Cambridge >U.K. >PC120@UK.AC.CAMBRIDGE.BIOLOGY >When I have started enquiring, why not continue.... >Which algorithm is used to determine the isoelectric point in the >PeptideSort program? >Peter Carlsson, Biochemistry, Cambridge, UK.