ARF6@manvax.bitnet ("FRANCES M. CARDILLO") (04/29/91)
SHORT COURSE IN BASIC TISSUE CULTURE
presented by
The Biotechnology Program
Manhattan College/College of Mt. St. Vincent
Bronx (Riverdale), NY
JUNE 3 - 6, 1991
The essential aspects and contemporary methods of growing
cells in culture will be presented mainly by hands-on procedures
and some demonstrations. There will be lectures on the cultured
cell, monolayer and suspension culture systems, cryogenic preser-
vation, primary cell culturing, contemporary methods of cell
identification, and cell nutrition. Hands-on procedures will
include pipetting, media preparation and filtration, the hemocy-
tometer method of cell counting, cryogenic preservation, cultur-
ing anchorage dependent cells, primary cell culturing, cell
identification using immunohistofluoresence, and karyotyping.
Demonstrations on the use of cryogenic equipment, various types
of culture vessels, two dimensional gel electrophoresis of cul-
tured cell proteins emphasizing labeling, isoelectric focusing,
polyacrylamide gel electrophoresis and autoradiography will also
be presented.
GUEST FACULTY
John Ryan
University of Connecticut
Dr. Peter Del Vecchio
Albany Medical College
Dr. James Haley
College of Mt. St. Vincent/
Einstein College of Medicine
For additional information contact the Course Director:
Frances M. Cardillo, Department of Biology, Manhattan College
Mount St. Vincent Campus, Bronx, New York 10471
PHONE: 212-549-8000 ext367 FAX: 212-549-0915
Bitnet: ARF6@MANVAX.BITNET