ARF6@manvax.bitnet ("FRANCES M. CARDILLO") (04/29/91)
SHORT COURSE IN BASIC TISSUE CULTURE presented by The Biotechnology Program Manhattan College/College of Mt. St. Vincent Bronx (Riverdale), NY JUNE 3 - 6, 1991 The essential aspects and contemporary methods of growing cells in culture will be presented mainly by hands-on procedures and some demonstrations. There will be lectures on the cultured cell, monolayer and suspension culture systems, cryogenic preser- vation, primary cell culturing, contemporary methods of cell identification, and cell nutrition. Hands-on procedures will include pipetting, media preparation and filtration, the hemocy- tometer method of cell counting, cryogenic preservation, cultur- ing anchorage dependent cells, primary cell culturing, cell identification using immunohistofluoresence, and karyotyping. Demonstrations on the use of cryogenic equipment, various types of culture vessels, two dimensional gel electrophoresis of cul- tured cell proteins emphasizing labeling, isoelectric focusing, polyacrylamide gel electrophoresis and autoradiography will also be presented. GUEST FACULTY John Ryan University of Connecticut Dr. Peter Del Vecchio Albany Medical College Dr. James Haley College of Mt. St. Vincent/ Einstein College of Medicine For additional information contact the Course Director: Frances M. Cardillo, Department of Biology, Manhattan College Mount St. Vincent Campus, Bronx, New York 10471 PHONE: 212-549-8000 ext367 FAX: 212-549-0915 Bitnet: ARF6@MANVAX.BITNET