SYEH@BIONET-20.BIO.NET (Spencer Yeh) (08/27/88)
Dear Bionet Users,
Kevin Beadles from the University of Calif. at San Francisco has
provided his IBM PC OligoMutantMaker program for distribution through
the BIONET lending library of contributed software. The program will
not be available for downloading because of the size of the program
files.
DESCRIPTION
OligoMutantMaker simplifies the designing and screening of
oligonucleotide-directed single amino acid substitution experiments by
searching for nucleotide sequences which introduce a restriction
endonuclease recognition sequence into the codon substitution site of the
mutant.
The program utilizes the redundancy of the genetic code to generate all
possible nucleotide sequences for a given amino acid substitution
(including nucleotide sequences in which silent mutations are introduced
into the 5' and/or 3' codons immediately adjacent to the substitution site)
and determines whether any restriction endonuclease recognition
sequences are present. Any nucleotide sequence containing a restriction
site is displayed/printed along with relevant information about the site
such as its restriction enzyme(s), the random frequency of the enzyme's
recognition sequence(s), the prototype of the enzyme, isoschizomers of the
enzyme, and the unit cost of the enzyme from various biochemical
suppliers.
CONTACT ADDRESS
For questions about the program or suggestions for future improvements
please contact:
Kevin Beadles
1044 1/2 Shrader St.
San Francisco, CA 94117
tel.: (415) 759-0148
(Kevin will call you back collect if he must return your call.)
SYSTEMS SUPPORTED
An IBM compatible computer running MS-DOS is needed.
AVAILABILITY
OligoMutantMaker is available from the BIONET Lending Library. Please
send a stamped, self-addressed return envelope along with a formatted
diskette with your request to:
BIONET Administrator
BIONET
700 East El Camino Real
Mountain View, CA 94040
tel.: (415) 962-7300
FURTHER INFORMATION
For BIONET subscribers, more information about the program, including
sample output, is available by typing HELP OLIGOMUTANTMAKER at the
BIONET @ prompt. (This help information is contained in the file
BC:OLIGOMUTANTMAKER.HLP).
Sincerely,
Spencer Yeh ARPAnet: bionet@bionet-20.arpa
Applications Analyst (for BIONET issues)
BIONET
(415) 324-4363
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