mfarrall@mrc-crc.ac.uk (Martin Farrall) (08/03/89)
POSTDOCTORAL POSITION IN MAMMALIAN DEVELOPMENTAL MOLECULAR GENETICS Most developmental malformations in humans have complex aetiologies that are poorly understood. However, several rare 'single gene' syndromes have been identified which are amenable to 'reverse genetic' strategies. A phenotypically similar developmental mutation in humans and mice that maps to homologous chromosomal segments has been described, Greig cephalopolysyndactyly syndrome (GCPS, human chromosome 7p13) and extra-toes (Xt, proximal mouse chromosome 13). GCPS has been localized by cytogenetic analysis of translocation breakpoints and linkage analysis, Xt has been mapped by linkage to proximal chromosome 13 markers. We propose to localize the GCPS translocation breakpoints within a long range restriction map, chromosome walk and/or jump towards the breakpoint and identify potential coding sequences. Progress towards this goal will be monitored by genetic mapping of conserved sequences in mice using interspecific crosses segregating for Xt. Candidate genes will be cloned for expression and sequencing studies. Subsequent gene analysis (in situ expression studies and transgenic mice to confirm the identity of 'candidate' genes) will dissect the molecular basis of these malformation syndromes. I have recently received funding from the Medical Research Council (UK) to commence this 'reverse genetic' project aimed at cloning the gene(s) mutated in GCPS and Xt (over 5 years). I am looking for a postdoctoral research assistant to make a committed contribution to this project, a training in molecular genetics is essential. Work will include 'HTF island' cosmid library construction, pulse field mapping and cDNA library construction and screening. If you are interested in further details please contact: Martin Farrall, Division of Molecular Medicine, Clinical Research Center, Northwick Park Hospital, Watford Road, Harrow, Middlesex, HA1 3UJ, UK. Telephone: +44 1 422 1442 FAX: +44 1 423 1275 E-mail: mfarrall%uk.ac.crc@ac.uk JANET/EARN/BITNET
PSHARP@vax1.tcd.ie (01/02/90)
POSTDOCTORAL POSITION ---- MOLECULAR BIOLOGY / MOLECULAR EVOLUTION at BAYLOR COLLEGE OF MEDICINE and UNIVERSITY OF TEXAS at the TEXAS MEDICAL CENTER, in HOUSTON Qualification: a Ph.D. degree or equivalent, with experience in gene cloning and sequencing work. Successful applicant will work on joint project headed by Dr. Larry Chan (Departments of Cell Biology and Medicine, Baylor College of Medicine) and Dr. Wen-Hsiung Li (Graduate School of Biomedical Sciences, U. Texas). Objective is to clone and sequence apolipoprotein genes from various mammals. Bench work conducted in Dr. Chan's laboratory. The Postdoc may pick up statistical analysis of DNA sequence data from Dr. Li, if he or she likes to do so. Annual salary: $20,000 or higher depending on qualification. contact : Wen-Hsiung Li Genetics Centers, G.S.B.S., University of Texas, P.O. Box 20334, Houston, Texas 77225 telephone: 713-792-4619 bitnet: gsbs005@utsph.bitnet
mfarrall@mrc-crc.ac.uk (Martin Farrall) (05/30/90)
POST-DOCTORAL RESEARCH ASSISTANT HUMAN DEVELOPMENTAL MOLECULAR GENETICS A postdoctoral research post is now available in the Division of Molecular Medicine to investigate human genes with roles in the control of embryogenesis. 'Reverse genetic' techniques will be used to isolate and clone the gene mutated in Greig cephalopolysyndactyly syndrome (GCPS), a condition with characteristic abnormal craniofacial and limb morphogenesis. Applicants must have received a training in molecular genetic techniques, applications from students who are 'writing up' are welcomed. For informal enquiries, please contact Dr. Martin Farrall (Tel. 081 869 3244, FAX 081 422 1442) Clinical Research Center, Northwick Park Hospital, Watford Road, Harrow HA1 3UJ, UK)