BRUSCHI@BIONET-20.BIO.NET (Carlo V. Bruschi) (01/05/89)
POSTDOCTORAL POSITION
To study the cell-cycle regulation of a cloned cell-division-cycle CDC6 gene
required for correct DNA synthesis and chromosome segregation in the yeast
Saccharomyces cerevisiae. Ph.D. in one of the following: Molecular Biology,
Genetics, Microbiology or Biochemistry with laboratory experience in molecular
biology and genetic engineering technology. Salary and benefits nationally
competitive. Send application, with curriculum vitae and names and addresses
of three references to:
Dr. Carlo V. Bruschi
Biotechnology Program
Department of Microbiology & Immunology
East Carolina University School of Medicine
Greenville, NC 27858-4354
(919) 551-3131 FAX: (919) 551-2012
BIONET Address: Bruschi@BIONET-20.BIO.NET
Federal law requires proper documentation of identity and employability at the
time of employment. It is requested that this documentation be included with
your application. AA/EEO.
-------smithj%ewald.decnet@BILBO.BIO.PURDUE.EDU ("EWALD::SMITHJ") (01/24/89)
Postdoctoral position A postdoctoral position is available in an X-ray crystallography laboratory to work on the determination of the three-dimensional structure of glutamine PRPP amidotransferase. The project has components of both crystallography and molecular biology. Crystals of diffraction quality have recently been obtained and the structure analysis is underway. Design and production of site-directed mutants and improvements to an existing expression system are needed for future structural studies. Candidates with crystallographic experience and an interest in molecular biology are preferred, although those with a solid background in biochemistry and a strong interest in molecular structure will be considered. The crystallography laboratory is well equipped with an area- detector diffractometer, rotating anode X-ray generators, X-ray cameras, molecular graphics workstations, film scanning facility and an excellent local computing and supercomputing environment. The molecular biology laboratory is fully equipped and includes access to facilities for oligonucleotide synthesis and protein sequencing. Candidates should send a curriculum vitae and names and addresses of at least two references. Contact: Dr. Janet L. Smith, Dept. of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA, (317)494-9246, SMITHJ@EWALD.BIO.PURDUE.EDU (internet), SMITHJ@PURCCVM (bitnet), or Dr. Howard Zalkin, Dept. of Biochemistry, Purdue University, West Lafayette, IN 47907, USA, (317)494-1618.
toms@ncifcrf.gov (Tom Schneider) (03/16/89)
I am looking for a hard-working postdoc with a degree in molecular
biology to do bench work on an exciting project in my lab.
PROJECT DESCRIPTION:
Binding sites, such as the lac operator on DNA or ribosome binding
sites on RNA, consist of a pattern to which a specific
macromolecule---a ``recognizer''---binds. These patterns are often
described by using a consensus sequence. Another way to characterize
them is to determine how much information is needed to specify the
pattern. For example, ribosome binding sites in E. coli can be
described using 11.0 bits of information. (A bit of information is the
information you would need to distinguish between two equally likely
events, such as a coin flip.) The number 11 is not meaningful by
itself; we need something to compare it to. Given the size of the
genome and the number of binding sites, we can calculate how much
information the recognizer needs to find the sites. For ribosomes in
E. coli this is 10.6 bits. This means that the amount of information
stored in the patterns at binding sites (11.0 bits) is just enough for
the sites to be found in the genome (10.6 bits).
The patterns at bacteriophage T7 promoters are a big exception. They
contain about 35 bits, although only 17 bits should be needed by the
RNA polymerase. If the polymerase really only uses half of the pattern
information, then we should be able to destroy the other half and still
have strong promoters. I synthesized, cloned and sequenced many strong T7
promoters with several basepair changes each, and showed that the
polymerase uses only 18+/-2 bits. This confirms the hypothesis that
the information in the pattern used by a recognizer can be predicted
from the selection job that has to be done to locate the sites in the
genome. The ``extra'' information in the phage genome is probably the
binding site of another protein.
The postdoc will continue bench work on this project. He or she must
have experience in molecular biology, including cloning, sequencing and
bacterial genetics. A knowledge of computers and information theory is
not required. The molecular biology and computer facilities here are
excellent.
Two papers that describe the project are:
T. D. Schneider, G. D. Stormo, L. Gold, and A. Ehrenfeucht.
Information content of binding sites on nucleotide sequences.
J. Mol. Biol., 188:415--431, 1986.
T. D. Schneider and G. D. Stormo.
Excess information at bacteriophage T7 genomic promoters detected
by a random cloning technique.
Nucleic Acids Research, 17:659-674 1989
STARTING DATE: immediate
LOCATION:
National Cancer Institute
National Institutes of Health
Frederick, MD
KIND OF POSITION: IRTA fellowship
NIH Intramural Research Training Award
1 year initial appointment, renewable for 3 years.
SALARY:
Years of Postdoctoral Experience upon entrance to duty
determines the initial Stipend:
initial 2nd year 3rd year
0 - 1 $20,000 21,500 23,000
1 - 2 $21,500 23,000 24,500
2 - 3 $23,000 24,500 26,000
The following application documents are required:
a. Curriculum Vitae
b. Bibliography
c. 3 letters of reference emphasizing research potential
d. Applicant's statement of research goals (approximately 1 page
in length), and type and purpose of training desired
e. Official copy of doctoral degree
f. Official copy of undergraduate and graduate or medical school transcripts
g. Brief summary of doctoral dissertation
h. SF-171 Application
Thomas Schneider
National Cancer Institute
Frederick Cancer Research Facility
Building 469, Room 144
P. O. Box B
Frederick, MD 21701
(301) 698-5581 (-5532 for messages)
computer network address: toms@ncifcrf.govLASKY@BIONET-20.BIO.NET (STEPHEN R. LASKY) (06/07/89)
POSTDOCTORAL POSITION AVAILABLE
We have an opening for a qualified postdoc in a well funded
molecular biology laboratory at Roger Williams General Hospital and
Brown University School of Medicine in Providence, Rhode Island. The
primary goal of the research will be to investigate the mechanism of anti-
proliferative action of the seco-steroid hormone, vitamin D. The cell line
that we are working with was developed at this hospital and therefore of-
fers some exclusiveness for these studies.
The project will include investigations into the effects of vitamin
D on steroid hormone receptor function, oncogene expression, tyrosine
phosphorylation, and other signal transduction systems. The candidate
should have a strong background in molecular biology or biochemistry.
The appointee will have the freedom to develop related projects and
pursue funding after the first year.
To apply for this position, send a CV with the names of three
references to S. R. Lasky PhD, Roger Williams General Hospital, 825
Chalkstone Avenue, Providence, RI 02908, or reply by e-mail to my
bionet address: lasky@bionet-20.bio.net.
Roger Williams General Hospital is and Affirmative Action and
Equal Opportunity Employer and encourages applications from members
of protected groups.
srLASKY
*********
-------smgxt01@mvs.ulcc.ac.uk ("Tom Salt, Inst Ophthalmology, LONDON") (10/20/89)
DEPARTMENT OF VISUAL SCIENCE
INSTITUTE OF OPHTHALMOLOGY (University of London)
Judd Street, LONDON WC1H 9QS, England
Post-Doctoral Research Assistant
Applications are invited for the post of Post-Doctoral Research
Assistant (Grade 1A) to work on a three year project, funded by the
M.R.C., entitled TRANSMITTERS OF VISUAL AND SOMATOSENSORY AFFERENTS
TO THE MAMMALIAN SUPERIOR COLLICULUS. Experience of neuro-
physiological and/or neuro-pharmacological techniques (especially
in relation to amino acid transmitters or sensory systems) would
be an advantage, although not necessarily essential. Salary is
in the region of 13000 to 14000 pounds Sterling.
Applications, including C.V. should be sent to: Dr T E Salt, at the above
address.
Informal enquiries are welcome.
Tel + 44 1 387 9621 ext 231.
Janet: smgxt01@uk.ac.ulcc.mvs
BitNet: smgxt01%mvs.ulcc.ac.uk@ukacrlbashford@scripps.edu (Don Bashford) (12/21/90)
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A postdoctoral fellowship is available to a candidate
interested in research protein electrostatics. Macroscopic
dielectric models of the protein-solvent system are being
developed. Applications can be made to titration and redox
properties of side-chains and prosthetic groups, protein
stability, solubility, electron transfer, proton transfer,
substrate binding affinity or electrostatic steering of
substrates by enzymes. Candidates should have a background
in phyisics, chemistry, biochemistry or biophyisics, and
should have some experience in computer programming.
The Department has a strong commitment to both
experimental and theoretical research in the structural
basis of molecular biology. It includes several research
groups in x-ray crystallography and NMR of proteins;
theoretical groups working on protein structure, dynamics,
energetics and folding; and researchers developing
techniques and software for computer graphics of biological
molecules. Facilities include a large number of advanced
computing workstations, a graphics laboratory, and Convex
and Cray supercomputers.
Candidates should send a CV, a statement of their
research interests and the names of three references to
Dr. Donald Bashford
Department of Molecular Biology
Research Institute of Scripps Clinic
10666 North Torrey Pines Road
La Jolla, California 92037
USA
Electronic mail: bashford@scripps.edu
phone: (619) 554-9612
FAX: (619) 554-8841
The Research Institute of Scripps Clinic is an equal
opportunity employer.jamesou@liverbug.hsc.usc.edu (James Ou) (05/17/91)
A postdoctoral position is available immediately to study
the molecular biology of hepatitis B virus. Projects include:
gene regulation, protein transport and oncogenesis. The
followings are a few recent publications from this laboratory:
1. Yeh et al. (1990) The arginine-rich domain of hepatitis B
virus precore and core proteins contains signal for nuclear
transport. J. Virol. 64: 6141-6147.
2. Guo et al. (1991) Leaky transcription termination produces
larger and smaller than genome size hepatitis B virus X gene
transcripts. Virol. 181: 630-636.
3. Yeh & Ou. (1991) Phosphorylation of hepatitis B virus precore
and core proteins. J. Virol. 65: 2327-2331.
4. Seto et al. (1988) Trans-activation of the human
immunodeficiency virus long terminal repeat by the hepatitis
B virus X protein. Proc. Natl. Acad. Sci. USA 85: 8286-8290.
If interested, please contact:
J.-H. James Ou, Ph.D.
Department of Microbiology
University of Southern California
School of Medicine
2011 Zonal Ave., HMR-401
Los Angeles, CA 90033
Tel: (213) 342-1720
Fax: (213) 342-1721
email: jamesou@zygote.hsc.usc.edu