PC_1@biology.cambridge.ac.uk (12/04/90)
Hello out there, Can anyone help me with telling me where to find an accurate nt sequence of lambda gt11...... Probably due to ageing and bad eyes, I've been unable to locate anything apart from the KpnI-SacI fragment in the databases. My apologies if you already have read this on the "BIONEWS" board, but the response there was very limited (.eq. none), so I give it a go here..... Peter Carlsson, Dept. of Biochemistry Univ. of Cambridge, UK pc_1@uk.ac.cambridge.biology
pgil%histone@LANL.GOV (Paul Gilna) (12/08/90)
I may well stand to be corrected on this but..... To the best of our knowledge, the complete sequence of gt11 has never been published. The vector was basically constructed by tacking various expression and promotor sequences to a variant of lambda gt10, which itself was a construct, etc, etc, Traditionally GenBank has not reflected construct sequences unless those sequences were sequenced to confirm the construction: while this is easily done for plasmid sequences, it is unlikley that this would normally be done for lambda as it is rather large; if I remember from my wet lab days, gt11 is about 9kb. It is possible that VecBase at MIT has it. Cheers, Paul Gilna, GenBank, Los Alamos
cherry@island.uucp (Mike Cherry) (12/09/90)
In article <9012071849.AA00831@histone.lanl.gov> pgil%histone@LANL.GOV (Paul Gilna) writes: > > It is possible that VecBase at MIT has it. > Just to save you some time looking for lambda sequences, VecBase contains no lambda phage vectors. The closest thing to a lamdba vector is the Pi-VX plasmid. Oh and by the way VecBase is not being kept updated, the last revision was August 1987. There are many commonly used vectors that have not been assembled into a sequence entry in any of the public databases. I guess there is a place for an entrepeneurial sequencer. Mike Cherry