CLBMSK@paterson.christie-hospital.manchester.ac.uk (07/17/89)
Manchester 17.07.1989
To the EMBL database staff
We submited a paper to Nucleic Acids Research and were ask for
an EMBL accesion number. We would like to send you the following sequence.
Could you please send the accesion number to UC.AK.MAN.CR.PICR::CLBMSK or
to Dr J. Heighway Department of Cancer Genetics, Paterson Institute for
Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX,
UK?
Sincerely yours
M. Santibanez Koref
DT 17-AUG-1989
XX
DE fragment of genomic human DNA showing biological activity when
DE expressed in E. coli.
XX
KW supercoiling; autoagglutination.
XX
OS Homo sapiens (man, homme, Mensch).
OC Eukaryota; Metazoa; Chordata; Vertebrata; Tetrapoda; Mammalia;
OC Eutheria; Primates.
XX
RN [1]
RA Heighway J., Geurts van Kessel A.H.M.;
RT "Isolation of a human genomic fragment, co-amplified with
RT c-Ki-ras, that affects plasmid supercoiling in E. coli";
RL Nucl. Acids Res. 15: 3411-3420 (1987).
XX
CC The sequence was co-amplified with c-Ki-ras in a lung tumor.
CC It is localised to the short arm of chromosome 12. Its
CC transcription leads to changes in the degree of supercoiling of
CC plasmids in certain E. coli strains and/or to autoagglutination
CC of the bacterial cells.
XX
SQ Sequence 369 bp; 124 A; 74 C; 95 G; 76 T;
GTACCAGGCA GCACAGAAAC AAGTTCTGGT AAACCATTGT TGCCATCAGG GTTATGTGTG
TAACAAGAAA TCTCATGAGG AGAGAAATCT CAGTTTCCTT CCCTGAGAAA AGAAAGGAGC
AAGAAAGGGA AGGGAAGAGT GGTTATAATG CCTTCAGGGT GCCCAGAAGA AAGAGAAGCC
CTTGGACAAT GAGGAAATCA AATCTCCATC TGCCTCAGAT GATGCTGGTA ACATCCGGAG
CCCCAAGGAA AGGGGCCCTT CACTTCCTGC TTGAAACATG ATAGATTTCC AGTGCCAAGT
GTCCTGACCA AGTATTTTGT GGCAAAGATA AAAGACAGGA GGAAATGGGT CAAAAACCCC
AGTGTAGAC
//