craigw@aecom.YU.EDU (Craig Werner) (06/29/88)
This is a relatively more complicated map. It is not only a map
of Charon 27 with the viability limit demarcated. It also has the
restriction sites indicated in the original publication. Furthermore,
span lines have been added to note the actual lengths of the restriction
fragments.
I refer to this map constantly when mapping Charon 27 clones.
%%
0.5 setlinewidth
%% Charon 27 follows
50 percentBLACK
0.4 inch LEFTMARGIN
8.1 inch RIGHTMARGIN
5 inch YCENTER
%%
8 POINTSIZE
/Times-Roman SETFONT
41783 PHAGELENGTH
0.3 inch ticLENGTH
0.4 inch textLENGTH
0 inch WIDTH
0 19700 linSEGMENT
(BglII 482) 482 linRESITEvd
(KpnI 17290) 17290 linRESITEv
(KpnI 18812) 18812 linRESITEv
(L-vl 19700) 19700 linRESITEv
0.1 inch WIDTH
19700 33552 linSEGMENT
(KpnI 32525) 32525 linRESITEv
(EcoRI 21569) 21569 linRESITEv
0.5 inch ticLENGTH 0.6 inch textLENGTH
(BamHI 22703) 22703 linRESITEv
0.3 inch ticLENGTH 0.4 inch textLENGTH
(HindIII 23513) 23513 linRESITEv
(XhoI 29654) 29654 linRESITEv
(SalI 33547) 33547 linRESITEv
(BglII 22784) 22784 linRESITEvd
(BglII 28898) 28898 linRESITEvd
(BglII 33256) 33256 linRESITEvd
(BglII 29780) 29780 linRESITEvd
0 inch WIDTH
33552 TotalLength linSEGMENT
% (R-vl 33552) 33552 linRESITEv
(KpnI 34182) 34182 linRESITEv
(BglII 35131) 35131 linRESITEvd
(***) 35146 linRESITEv
0.3 inch textLENGTH
9 POINTSIZE
/Times-Roman SETFONT
(Viability) 26000 linLABEL
(Limit) 26000 linLABELd
%
%% End of Map
%% Span lines follow
0.05 inch WIDTH
0.15 inch textLENGTH
7 POINTSIZE
/Times-Roman SETFONT
/ds 2 def
-1.5 inch spanDISTANCE % spanDISTANCE and spanLENGTH synonyms
% BglII fragments
0 482 linNSPANd
482 22784 linNSPANd
22784 28898 linNSPANd
28898 29780 linNSPANd
29780 33256 linNSPANd
33256 35131 linNSPANd
35131 41783 linNSPANd
2.0 inch spanDISTANCE
% KpnI fragments
0 17290 linNSPAN
17290 18812 linNSPAN
18812 32525 linNSPAN
32525 34182 linNSPAN
34182 41783 linNSPAN
1.5 inch spanDISTANCE %
%EcoRI/Hind III fragments
0 21569 linNSPAN
21569 22703 linNSPAN
22703 23513 linNSPAN
23513 41783 linNSPAN
--
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Craig Werner (Lognames: werner, craigw)
"This is no social crisis, just another tricky day for you."