annala@neuro.usc.edu (A J Annala) (12/10/88)
I would appreciate receiving information, pseudo code algorithms, and/or actual source code that would enable me to assemble a single large image from a series of overlapping images (512 X 384 pixel) acquired from NTSC video rate 8 bit CCD camera. Ideally, a least squares minimization code should enable me to accomplish the registration process. However, I am open to examining any other approach to solving this problem. It seems to me that astronomers working on assembling mosaic images of the moon, other planets, and their satellites should have this problem pretty well solved. In addition, people assembling LANDSAT and/or SPOT earth resources satellite images should have workable code. My images are even simpler than these space based applications. I will be acquiring overlapping NTSC video frames from brain sections examined in a Zeiss Axiophiot light microscope. The images will be preprocessed to remove artifacts induced by nonuniformities in illumination of the microscope field, dark current, etc. I will acquire the images normal to the plane of section. No orbit skewing, sun shadow angle, or other similar distortion should be present in the starting images. However, the least squares (or other registration algorithm) will have to work with roughly 1.0 OD unit dynamic range continuous grey scale images to accomplish the registration process. Please email responses to: annala%neuro.usc.edu@oberon.usc.edu Thanks, AJ Annala, USC NeuroScience Program