861033h@aucs.uucp (HOGIE) (10/28/89)
I am trying to do a Scanning Electron Micorscopic examination of microbial floura of deciduous rhizosphere and rhizoplane and have encountered problems with technique. Without the use of a critical point drier (ours is broken), I have tried coating unwashed and washed roots in an attempt to actually see some of the numerous bacteria present. But this is just leaving me more frustrated and logging hours of vertually wasted time on the SEM. There is just too much particulate matter obstructing the view. I have one more idea left - sonic the roots free of these particles but after that I'm stuck. Is there anyone out there who has developed a SEM procedure for the fixation and coating of rhizosphere and rhizoplane that gives reasonable results and doesn't use a critical point drier?? I would appreciate any ideas, comments, or references. -- Michael Hogan Address: c/o Biology Department Internet: 861033h@AcadiaU.CA Acadia Univeristy Bitnet: 861033h@Acadia Wolfville, Nova Scotia Phone: (902) 542-1557 B0P 1X0