snell@utzoo.uucp (snell) (03/01/88)
I do not know why centrifuge protocols are so commonly described with rpm's rather than g-force equivalents, since the rpm does not directly translate to anything (it all depends on the effective radius of the particular rotor head...). Any ideas on why this is the custom (I could provide a stack of current examples, if you doubt it is the norm)? So, I want to reproduce someones centrifuge protocol. They used a Sorval RC5, rotor SS34. What would be the effective radius for the distal tip of a centrigue tube in such a rotor head? (I can then work out the g-force and do it in our Beckman--of course, I only be able to get a ballpark figure, as they did not say what sort of microtube they used). All that was given was 20000 rpm (grrrr.). Thanks -- Name: Richard Snell Mail: Dept. Zoology, Univ. Toronto Toronto, Ontario, Canada M5S 1A1 UUCP: {allegra,decvax,ihnp4,linus,pyramid,yetti,utai}!utzoo!snell