[net.bio] Digital analysis of microscopic images

kovish@mprvaxa.UUCP (Barrie Kovish) (03/06/84)

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Heres a "BioComputing" idea I have wondered about for 
a while. Suppose we take a film of some cellular behavior
under an optical microscope. This film would then be digitized
and processed by a computer. A fourier transform of each image 
location intensity (color?) as a function of time would be 
performed. The computer could then insert artificial colors
into the image depending upon the time dependent fluctuations 
in intensity at each image location.  More complicated functions
of the intensity fluctuations might be developed to color the
image. Would the resulting image reveal aspects of cellular
behavior not observable normally? Some reasons why it might are:
small or quick intensity fluctuations will probably be integrated
by the eye and hence invisible; objects too small to be resolved 
by the optical microscope may still manifest themselves optically by 
producing intensity fluctuations (only marginally similar to light 
scattering studies of macromolecules). Has any one tried this? 
I'm curious what the results were/would be. Thanks for any info.